Small-Molecule Hapten Design and Synthesis Are Available in Several Technology Service Companies
In recent years, food addictive abuse has aroused public concerns since it will jeopardize human health to a great extent. Governments of most countries have issued strict regulations on addictive use in the food industry, which requires a set of technical supports to conduct detection.
Creative Biolabs, one of the top service companies in the field of small-molecule antibody development, has launched several hapten studies, and eventually yields some achievements. Until now, the company is qualified to provide support for the design and synthesis of small-molecule haptens for various compounds such as food additives.
Haptens are extensively used in many areas especially in the development of anti-hapten antibodies. To produce a class- or compound-specificity antibody, designing a suitable hapten is always a critical step. Haptens, recognized as small molecules with a low molecular weight of less than 2-5 kDa, are unable to trigger an immune response because they lack epitopes. The small molecules become immunogenic unless conjugated with suitable carrier macromolecules such as proteins. Therefore, several factors including the design, modification and synthesis of haptens, selection of the appropriate carrier protein, and the method of conjugation determine the successful production of desired antigen-special antibodies.
As the core determinant of anti-hapten antibody development, hapten requires careful design, modification, and synthesis. In order to receive the optimal hapten design, criteria are set based on the following factors: the location of the conjugation, length of handles, type of spacer arms, the size of haptens, etc. All of these show abundant possibilities of hapten modification through which researchers are competent to design ideal antibodies. After design and modification, scientists, by utilizing various approaches, through several synthetic pathways, can prepare the redesigned haptens into a stable form as is called the synthesis of haptens.
It is worth noting that even though the haptens have been synthesized, the whole process needs to be carried on. Haptens must be conjugated to a larger carrier protein so as to induce the greatest immune response. Meanwhile, the proteins require thorough evaluation due to their complex characteristics. In addition, researchers are supposed to select the optimal method of coupling a hapten. Due to the heavy workload, researchers generally need help from some companies specialized in the field. In this case, Creative Biolabs and their competitive counterparts gradually emerge to be indispensable assistants.
Once the step of conjugation is accomplished, researchers are ready to obtain the artificial antigen, which may contain un-reacted haptens, salts, or other small molecules. The technology supporters mentioned above still play a crucial role in purifying the redundant substances by dialysis and gel chromatography methods. At last, they can also help to identify the purified haptens qualitatively and quantitatively.
To facilitate antibody research and development, several prominent technology supporters like Creative Biolabs provide hapten design and synthesis of high quality.
"These companies are reliable to be entrusted the task of hapten design and synthesis because of their first-class expertise," said a distinguished researcher specialized in antibody study.
